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Hard to transfect and sensitive primary cardiomyocytes were infected with GFP expressing Amplicon vectors at a multiplicity of infection (MOI) of 1 to 50 particles per cell.

The GFP protein expression was directly visualized by fluorescence microscopy. The intensity of the green fluorescence correlated to the amount of the GFP protein produced after GFP gene transfer by the Amplicon vectors.

MOI = 0

High MOI increases GFP gene expression without cytotoxicity

MOI = 1

MOI = 5

MOI = 10

MOI = 50

Bioviron © 2017

BIOVIRON

Gene expression in primary cells

More applications

HSV amplicons are powerful vectors for gene transfer :

 

-  High efficacy of gene transfer in mammalian cells

-  Large payload gene transfer (up to 152 kbp)

-  Strong or regulated combinatorial gene expression

-  Homogeneous multigenic expression

-  Amplicon vectors are safe (no cytotoxicity)

-  Amplicon vectors are easy to manipulate

-  The gene transfer protocol is very simple

-  Amplicon vectors can be produced at high scale

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